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Table 2 Effect of the specific gene mutation on the fermentation characteristics at the dilution rate of 0.2 h-1

From: Catabolic regulation analysis of Escherichia coli and its crp, mlc, mgsA, pgi and ptsG mutants

Strains

Biomass conc. (g/L)

Glucose conc. (g/L)

Glucose uptake rate (mmol/g/h)

Acetate formation rate (mmol/g/h)

Biomass yield (g/g)

BW25113

1.45 ± 0.06

ND*

3.07 ± 0.13

ND*

0.37 ± 0.015

Δcrp

1.57 ± 0.07

ND*

2.83 ± 0.13

0.35 ± 0.018

0.39 ± 0.018

crp +

1.42 ± 0.07

ND*

3.12 ± 0.14

ND*

0.36 ± 0.018

Δpgi

1.6 ± 0.08

ND*

2.77 ± 0.14

ND*

0.4 ± 0.021

Δmlc

1.65 ± 0.08

ND*

2.69 ± 0.13

ND*

0.41 ± 0.019

ΔmgsA

1.55 ± 0.07

ND*

2.87 ± 0.14

0.1 ± 0.005

0.39 ± 0.018

  1. ND*: not detectable, where glucose detectable limit was 0.038 g/l.
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Microbial Cell Factories

ISSN: 1475-2859

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