Production of B. breve protein Bbr_0140. (A) SDS-PAGE analysis of soluble fractions of NZ9000 + pTX8048-0140 without nisin induction (lane1), and following induction with 0.2% (v/v) nisin (lane 2), soluble fraction of NZ9000 + pNZ8048-0140C following 0.2% nisin induction (lane 3) and soluble fraction of NZ9000 + pNZ8048-0140N following 0.2% nisin induction (lane 4). (B) SDS-PAGE analysis of purified Bb_0140 from cell extracts of NZ9000 + pTX8048-0140 following 0.2% nisin induction. Lanes: L, prestained marker; 1-3, elution fractions. (C) Immunoblotting analysis showing Bbr_0140 using mouse anti-polyhistidine antibody as primary antibody. Bbr_0140 is indicated by an arrow.