Figure 1From: Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression systemMaps and sequence overview of pTX8048 and pTX8049. (A) Vector maps of pTX8048 and pTX8049, which were constructed by insertion of the E. coli thioredoxin gene trxA, an enterokinase-specific cleavage site and a hexahistidine-tag as illustrated; (B) DNA sequence overview of the multiple cloning sites of pTX8048 and pTX8049. PnisA, nisin-inducible promoter; RBS, ribosome binding site; trxA, E. coli thioredoxin A-encoding gene; MCS, multiple cloning site; term, transcriptional terminator; Cm, chloramphenicol cassette. All restriction sites shown here are unique and can be used as cloning sites. The restriction site BamHI in pTX8048 and pTX8049 allows the in-frame cloning of the gene of interest.Back to article page