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Figure 3 | Microbial Cell Factories

Figure 3

From: Heterologous production of active ribonuclease inhibitor in Escherichia coli by redox state control and chaperonin coexpression

Figure 3

RI protein yields after shake flask EnBase fed-batch cultivation of E. coli ER2566 pET21bRI pGro7 without DTT (-DTT) or with addition of 12 mM of DTT 2 hours after induction of RI (+DTT). Total (white bars) and soluble (black bars) RI amounts in milligrams per gram of dry cell weight [mg (g CDW)-1] (A) and RI activities in normalised crude extracts in kilo units per gram of wet cell weight [kU (g CWW)-1] (B) 4 hours after RI induction. The amounts of target protein in (A) were determined from scanned SDS-PAGE gel images as described in material and Methods. Standard deviations represent RI amounts from 3 assays.

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