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Figure 3 | Microbial Cell Factories

Figure 3

From: Overproduction of Bacillus amyloliquefaciens extracellular glutamyl-endopeptidase as a result of ectopic multi-copy insertion of an efficiently-expressed mpr gene into the Bacillus subtilis chromosome

Figure 3

Cells plated on skim-milk test plates: The dependence of extracellular protease activity on the copy number of the integrated mprB.amy cassettes. 1.-clone of B. subtilis JE852; 2. and 3.-1 and 3 copies of mprB.amy-cassette integrated in the chromosome of JE852; 4.-clone of the plasmid-carrier strain B. subtilis JE852/pHE52mpr.

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