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Figure 1 | Microbial Cell Factories

Figure 1

From: Overproduction of Bacillus amyloliquefaciens extracellular glutamyl-endopeptidase as a result of ectopic multi-copy insertion of an efficiently-expressed mpr gene into the Bacillus subtilis chromosome

Figure 1

Structure of the pHE52 mpr plasmid carrying the mprB.amy cassette. P rp - promoter of the rplU-rpmA genes from B. amyloliquefaciens A-50; Ter - Rho-independent transcription terminator of the pheA gene. The Bgl II-site-ended (boldface) followed by 5'-portion of B. amyloliquefaciens A-50 mpr gene is shown. In this part, SD-sequence and ATG-codon are marked as boldface and capital letters, respectively. Two putative cre elements, which are homologous to the known [4547] consensus sequence, are also indicated.

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