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Table 3 Densitometric analysis of the fusion proteins from the Coomassie blue-stained SDS-PAGE gel (mg/L), as compared to ELISA results (mg/gdw)

From: Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

Construct name Fusion protein
(mg/L)**
Enfuvirtide peptide
(mg/L)*
Enfuvirtide peptide (mg/gdw)
pSECFliCFFuz 18.5 1.8 0.7
pNWFliCFFuz 32.9 3.3 1.3
pNWNCFFuz 61.0 13.4 2.8
pNWNFFuz 2.6 1.3 0.3
pNWCFFuz ND ND 0
  1. *Enfuvirtide peptide calculated as a percentage of total fusion protein.
  2. ** Results quoted as the average of three experiments.