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Table 3 Densitometric analysis of the fusion proteins from the Coomassie blue-stained SDS-PAGE gel (mg/L), as compared to ELISA results (mg/gdw)

From: Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

Construct name

Fusion protein

(mg/L)**

Enfuvirtide peptide

(mg/L)*

Enfuvirtide peptide (mg/gdw)

pSECFliCFFuz

18.5

1.8

0.7

pNWFliCFFuz

32.9

3.3

1.3

pNWNCFFuz

61.0

13.4

2.8

pNWNFFuz

2.6

1.3

0.3

pNWCFFuz

ND

ND

0

  1. *Enfuvirtide peptide calculated as a percentage of total fusion protein.
  2. ** Results quoted as the average of three experiments.
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Microbial Cell Factories

ISSN: 1475-2859

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