Table 2 List of primers, their corresponding nucleotide sequences and application
Primer name | Nucleotide sequencea | Application |
|---|---|---|
SpoIVF | CGGAAAGCTT GTTGGTGCAGTGAC | Spo0A |
SUpR | CTAGGATCC GTAAGAGGCTCCTAAATCAACCG | Spo0A |
SDoF | CTAGGATCC GCCATGGTAGCCGATAAGC | Spo0A |
SDoR | CTACCCGGG GATTAATCTGGGCAATGGTTGC | Spo0A |
UplytCF | CTCGGTACC CTTAGAGGAGGGATGAGG | LytCE |
UplytCR | CTCGGATCC ACGGATGACGGTCACCAA | LytCE |
DolytEF | CTCGGATCC TTGAACAAAGCCGCTGCA | LytCE |
DolytER | CTCCCCGGG ACGGCCGATGCCGCGTTA | LytCE |
FlgMF | GTTAGGTACC GATCATTGTCTAGATTG | FlgM |
FMNtR | CTGCTCGAG CATCTTCACCTCAATTTC | FlgM |
FMCtF | CTGCTCGAG CAAGTAATGAGAGGGGGCTC | FlgM |
FMCR2 | CATCCCGGG TTGAGCGTCACCGTAAC | FlgM |
ClpUp | GTAGGTACC CAGCAGGCTCTAAATGTTG | ClpP |
ClpUpR | GTAGGATCC TCGAGAATAAATGTCATACG | ClpP |
CDoF | GGATCC TCGATGAATGCGATAAGAG | ClpP |
CDoR | GATATC TGTCTATCTTGTCCGCTTG | ClpP |
LacF | CTAGGTACC AATCGCTCACATCTC | AprX |
AprXR1 | CGACTCGAG CTGTACCATGGAATATCC | AprX |
AprXF2 | CTACTCGAG CAGCAGAGGGTGCGATTC | AprX |
AprXR2 | CTACCCGGG GACGATTCCGAGATGAAGCAC | AprX |
σDKpn | CTCGGTACC CTCGCGTTACGCTCTTTCTGT | FliC |
FliCendR | CGACGGATCC GCACTCGAG ACGAAGTAATTGTAATAC | FliC |
FlagXhoF | GAGACTCGAG GAATCTGGAGCAGATTATA | FLAG tag |
FuzendR2 | GTCTAGGATCC TTAAAACCAATTCCATAATG | Enfuvirtide |
NRev | CTTAGTCGAC ACCTGCAGCATCGTCTCC | N-terminal FliC |
CFOR | CTACGTCGAC CGCTCTTACCTAGGAGCT | C-terminal FliC |
CstartR FuzF FuzR | GTCTGTCGAC CATTAAAATTTCCTCCTTG GCTGTCGAC ATGTATACGTCATTAATTCATTCATTAATTGAAGAATCACAAAATCAACAAGAAAAAAATGAACAAGAATTATTAGAATTAGATAAATGGGCATCATT ATGGAATTGGTTTATGTGGATCCTCG CGAGGATCC ACATAAACCAATTCCATAATGATGCCCATTTATCTAATTCTAATAATTCTTGTTCATTTTTTTCTTGTTGATTTTGTGATTCTTCAATTAAAGTATGAATTA ATGACGTATACATGTCGACAGC | C-terminal FliC Enfuvirtide Enfuvirtide |