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Strains
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B. halodurans BhFD05
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Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp
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Berger et al (2009)
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B. halodurans BhFDL05S
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Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE
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This study
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B. halodurans BhFDL05SC
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Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔclpP
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This study
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B. halodurans BhFDL05SM
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Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔflgM
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This study
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B. halodurans BhFDL06S
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Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔaprX
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This study
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E. coli DH10B
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(F- mcr A Δ(mrr-hsd RMS-mcr BC) (φ80dlac ZΔM15)Δlac X74end A1 rec A1 deo R Δ(ara-leu) 7697 ara D139 gal U gal K nup G rps Lλ-
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Invitrogen
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Plasmids
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pSEC194
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CmR, ApR, thermosensitive integration vector
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Crampton et al (2007)
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pNW33N
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E. coli/Bacillus/Geobacillus shuttle vector
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Bacillus Genetics Stock Centre, (BGSC) Ohio, USA
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pSECFliCFFuz
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pSEC194, σD promoter, hag gene (FliC1-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions
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This study
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pNWFliCFFuz
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pNW33N, σD promoter, hag gene (FliC1-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions
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This study
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pNWNCFFuz
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pNW33N, σD promoter, truncated hag gene (FliCΔ46-201), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions
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This study
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pNWNFFuz
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pNW33N, σD promoter, N- terminal hag gene (FliC1-45), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions
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This study
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pNWCFFuz
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pNW33N, σD promoter, ATG, C-terminal hag gene (FliC202-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions
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This study
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