Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system

Strain or plasmid Relevant characteristics Source or reference
Strains   
B. halodurans BhFD05 Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp Berger et al (2009)
B. halodurans BhFDL05S Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE This study
B. halodurans BhFDL05SC Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔclpP This study
B. halodurans BhFDL05SM Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔflgM This study
B. halodurans BhFDL06S Δhag, ΔwprA, ΔfliD, Δalp, Δapr, Δvpr, Δasp, Δspo0A, ΔlytCE, ΔaprX This study
E. coli DH10B (F- mcr A Δ(mrr-hsd RMS-mcr BC) (φ80dlac ZΔM15)Δlac X74end A1 rec A1 deo R Δ(ara-leu) 7697 ara D139 gal U gal K nup G rps- Invitrogen
Plasmids   
pSEC194 CmR, ApR, thermosensitive integration vector Crampton et al (2007)
pNW33N E. coli/Bacillus/Geobacillus shuttle vector Bacillus Genetics Stock Centre, (BGSC) Ohio, USA
pSECFliCFFuz pSEC194, σD promoter, hag gene (FliC1-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions This study
pNWFliCFFuz pNW33N, σD promoter, hag gene (FliC1-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions This study
pNWNCFFuz pNW33N, σD promoter, truncated hag gene (FliCΔ46-201), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions This study
pNWNFFuz pNW33N, σD promoter, N- terminal hag gene (FliC1-45), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions This study
pNWCFFuz pNW33N, σD promoter, ATG, C-terminal hag gene (FliC202-275), FLAG tag, enterokinase cleavage site and Enfuvirtide gene as in-frame fusions This study