Schematic representation of the flagellin fusion constructs containing different regions of the FliC protein fused to the FLAG tag and the Enfuvirtide peptide (Fuz) expressed in B. halodurans BhFDL05S. The numbers indicate the size (amino acids) of the different fragments of the FliC protein used for the different constructs. The different colour blocks indicate the different regions of the FliC protein: the full-length flagellin protein (FliC1-275), the N-terminal region (FliC1-45) in blue, the C-terminal region (FliC202-275) in yellow and a truncated version with 157 amino acids of the central variable region (grey) deleted (FliCΔ45-275). The red block indicates a FLAG tag and the green block the Enfuvirtide (Fuz) peptide. An enterokinase cleavage site was inserted between the FLAG tag and Enfuvirtide peptide.