1,3-Propanediol dehydrogenases in Lactobacillus reuteri: impact on central metabolism and 3-hydroxypropionaldehyde production

Table 5 Glucose and glycerol consumption and end-product formation in L. reuteri DSM 20016 and its mutant derivatives LFB1001 and LFB1002 grown with and without glycerol^a

Strain	Glucose (Consumption)	Glycerol (Consumption)	lactate	ethanol	1,3-PDO
without glycerol
DSM 20016 (wild type)	- 31.8 ± 3.6	n.a	36.6 ± 1.8	62.7 ± 7.5	n.d.
LFB1001 (Δlr_1734)	- 32.1 ± 3.6	n.a.	36.8 ± 3.0	62.8 ± 7.6	n.d.
LFB1002 (Δlr_0030)	- 34.2 ± 0.4	n.a.	38.6 ± 0.8	60.9 ± 5.8	n.d.
with 35 mM glycerol
DSM 20016 (wild type)	- 24.8 ± 1.8	- 34.5 ± 3.8	27.1 ± 2.5	20.5 ± 5.1	19.8 ± 0.8
LFB1001 (Δlr_1734)	- 24.8 ± 1.6	- 34.5 ± 4.8	26.4 ± 2.3	20.5 ± 3.7	19.1 ± 0.2
LFB1002 (Δlr_0030)	- 24.9 ± 2.6	- 34.5 ± 4.0	27.1 ± 2.8	32.2 ± 4.9^b	16.3 ± 0.5^b

^a Average values and standard deviations from duplicate experiments are given in mM. n.a.: not applicable, n.d.: not detected.
^b Letters above values indicate significant difference (p < 0.05) compared to the two other strains in a t-test.

ISSN: 1475-2859