Table 1 Strains and plasmids used in this study and their relevant characteristics

Strains

E. coli

DH5α

Cloning host

[37]

L. reuteri

DSM 20018

Wild type strain, human intestinal isolate

DSMZ^b

SD2112

Wild type strain, human milk isolate

ATCC^c

LFB1001

lr_1734 gene replacement (lr_1734::P₃₂cat) derivative of L. reuteri DSM20016, Cm^R.

This work

LFB1002

lr_0030 gene replacement (lr_0030::P₃₂cat) derivative of L. reuteri DSM20016, Cm^R.

This work

LFB1003

lr_1734 gene replacement (lr_1734::P₃₂cat) derivative of L. reuteri SD2112, Cm^R.

This work

Plasmids

pNZ5319

Cm^R, Em^R, gene replacement mutagenesis vector.

[25]

pLFB1001

Cm^R, Em^R, pNZ5319 derivative containing 1.1 kb 3' flanking region of lr_1734.

This work

pLFB1002

Cm^R, Em^R, pNZ5319 derivative lr_1734::cat gene replacement mutagenesis vector containing 5'- and 3'-flanking regions of lr_1734.

This work

pLFB1003

Cm^R, Em^R, pNZ5319 derivative containing 1.2 kb 5' flanking region of lr_0030.

This work

pLFB1004

Cm^R, Em^R, pNZ5319 derivative lr_0030:: cat gene replacement mutagenesis vector containing 5'- and 3'-flanking regions of lr_0030.

This work