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Figure 6 | Microbial Cell Factories

Figure 6

From: Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy

Figure 6

Quantification of extracelluar expression of rSbpA/Bet v1 by B. subtilis 1012. SDS-PAGE analysis of rSbpA/Bet v1 (10 μg) purified from E. coli (lane 1) and of rSbpA/Bet v1 isolated from 1 mg peptidoglycan-containing sacculi of Ly. sphaericus CCM 2177 after incubation with 20 ml culture supernatant of a B. subtilis 1012 culture harvested 3 h after induction of expression (lane 2).

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