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Figure 4 | Microbial Cell Factories

Figure 4

From: Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy

Figure 4

Electron micrograph of negatively stained preparations describing concentration and purification of the S-layer/allergen fusion protein rSbpA/Bet v1 from B. subtilis culture medium by recrystallization on solid supports. (A) Peptidoglycan-containing sacculus of Ly. sphaericus CCM 2177 without S-layer lattice. Bar = 100 nm (B) Peptidoglycan-containing sacculus of Ly. sphaericus CCM 2177 after first incubation with B. subtilis 1012 culture medium containing secreted rSbpA/Bet v1 monomers. A patch of recrystallized rSbpA/Bet v1 on the surface exhibiting the square lattice structure is clearly visible. Bar = 100 nm (C) Peptidoglycan-containing sacculus of Ly. sphaericus CCM 2177 after repeated incubation with B. subtilis 1012 culture medium containing secreted rSbpA/Bet v1 monomers. Bar = 100 nm (D) The ultrastructure of the closed rSbpA/Bet v1 monolayer on the solid support in detail. Bar = 50 nm.

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