Figure 2
From: Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system
Comparison of retinal production from various BCM(D)O genes. Retinal and β-carotene production and cell growth of E. coli harboring pT-HB, pT-HBblh, pT-HBbrp, pT-HBbrp2, pT-HBBCMO1, and pT-HBSR. The blh gene of Halobacterium sp. NRC-1, the brp gene of Halobacterium sp. NRC-1, the brp2 gene of N. pharaonis, the BCMO1 gene of M. musculus and the SR codon-optimized blh gene of the uncultured marine bacterium 66A03 were introduced into the β-carotene plasmid, pT-HB, resulting in pT-HBblh, pT-HBbrp, pT-HBbrp2, pT-HBBCMO1, and pT-HBSR, respectively. Culture was carried out in 2YT medium containing 0.5% (w/v) glycerol and 0.2% (w/v) arabinose for 48 hours at 29°C. Open bars and solid bars represent 24 hrs and 48 hrs, respectively.