Figure 4

Morphological change of Aspergillus niger SKAn1015 induced by osmolality. (A) Projected area as determined by automatic image analysis is shown at 10 different culture broth osmolalities. At standard osmolality of 0.4 osmol kg^-1 the pellet size was generally rather heterogeneous, this led to considerably higher standard deviation. (B) Display of corresponding shape descriptors, aspect ratio (grey), solidity (black) and circularity (white) as measured by image analysis. Values are means for at least three 2L cultivations. 100 fungal particles or more were analyzed. Pictures were made after 48 hours of cultivation with a magnification 32 ×. Automatic image analysis was conducted with ImageJ 1.44 m. Beyond 4.2 osmol kg^-1 a perfect mycelial growth was exhibited making a macro morphologic analysis with the introduced parameters impractical.