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Figure 1 | Microbial Cell Factories

Figure 1

From: Endotoxin-free purification for the isolation of Bovine Viral Diarrhoea Virus E2 protein from insoluble inclusion body aggregates

Figure 1

Protein analysis of pET-SUMO-E2-T1 expression in E. coli BL21 (DE3). (A) Protein from soluble and insoluble fractions separated by electrophoresis on 10% Bis-Tris gel and stained with Coomassie blue. Lane 1, SeeBlue® Plus2 MW standard; lane 2, soluble protein fraction at 0 hours; lane 3, insoluble protein fraction at 0 hours; lane 4, soluble protein fraction 2 hours post IPTG induction; lane 5, insoluble protein fraction 2 hours IPTG post induction; lane 6, E2-T1 purified from IB (0.4 μL); lane 7, E2-T1 purified from IB (2 μL). (B) Western blot image of E2-T1 protein expressed in E. coli BL21 (DE3). Equivalent amounts of protein from the soluble and insoluble fractions were transferred to Hybond C and incubated with an anti-his antibody and detected by ECL. Lane 1, soluble protein fraction 0 hours; lane 2, insoluble protein fraction 0 hours; lane 3, soluble protein fraction 2 hours post IPTG induction; lane 4, insoluble protein fraction 2 hours post IPTG induction.

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