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Figure 8 | Microbial Cell Factories

Figure 8

From: High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development

Figure 8

Purification of recombinant GST-opt-VP1 protein. (A) Chromatographic profile of GST-opt-VP1 protein eluted from a GSTrap FF affinity column. The cytosolic extract of E. coli strain BL21(DE3)-pLysS expressing GST-opt-VP1 protein was loaded into the GSTrap FF column and the bound protein was eluted with elution buffer as described in Material and Methods. (B) SDS-PAGE analysis of the eluted fractions collected from the GSTrap FF affinity column. Lane M, pre-stained protein marker; lane 1, flow through; lane 2, fraction obtained after column washing, lane 3 and 4, eluted fraction 1 and 2, respectively, collecting after column elution. (C) Identity of the GST-opt-VP1 protein determined by MALDI-TOF. The bold letters represent actual amino acid matches.

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Microbial Cell Factories

ISSN: 1475-2859

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