Figure 7From: High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine developmentDistribution of E. coli -expressed GST-opt-VP1 protein in the soluble and insoluble factions. SDS-PAGE (A) and Western-blotting (B) were performed to examine the solubility of the E. coli-expressed VP1 protein. Anti-GST tag monoclonal antibody was used to recognize the VP1 protein. Lane M, pre-stained protein marker; lane 1, soluble fraction; lane 2, insoluble fraction.Back to article page