Identification and characterization of a new true lipase isolated through metagenomic approach

Table 2 Effect of organic solvents, pH, NaCl and temperature on the stability of LipC12

Variable	Residual	Variable	Residual	Variable	Residual
	activity (%)		activity (%)		activity (%)
Solvent stability^a		Solvent stability^a		Sodium chloride^d
None^b	100.0 ± 5.7	DMF 15%	223.1 ± 12.5	None^b	100.0 ± 6.5
Methanol 15%	1204.1 ± 20.6	DMF 30%	355.9 ± 2.8	0.9 M	100.9 ± 8.5
Methanol 30%	1561.1 ± 72.4	Acetonitrile 15%	62.7 ± 1.9	1.9 M	102.2 ± 7.1
Ethanol 15%	587.6 ± 28.2	Acetonitrile 30%	316.2 ± 69.7	2.3 M	99.7 ± 5.0
Ethanol 30%	428.4 ± 39.1	pH stability^c		2.8 M	109.0 ± 11.7
1-Propanol 15%	329.0 ± 15.0	AC 3	0.0	3.2 M	99.1 ± 7.7
1-Propanol 30%	1161.9 ± 27.4	AC 4	83.9 ± 5.3	3.7 M	111.8 ± 6.8
2-Propanol 15%	170.6 ± 3.3	AC 5	85.7 ± 5.9	Thermostability^e
2-Propanol 30%	696.2 ± 57.9	AC 5.5	70.5 ± 4.0	0°C^b	100.0 ± 4.23
Glycerol 15%	167.8 ± 2.1	MES 5.5	77.9 ± 6.1	20.5°C	100.0 ± 1.0
Glycerol 30%	233.0 ± 6.4	MES 6	92.4 ± 5.3	30.0°C	96.2 ± 7.7
THF 15%	463.6 ± 139.4	MES 7	95.5 ± 1.0	40.0°C	100.0 ± 4.2
THF 30%	1690.9 ± 29.0	HEPES 7	95.7 ± 8.1	50.7°C	94.0 ± 6.4
Acetone 15%	66.5 ± 1.6	HEPES 7.5^b	100.0 ± 4.9	60.6°C	75.8 ± 4.0
Acetone 30%	624.2 ± 3.5	GLC 7.5	96.9 ± 5.1	70.0°C	64.9 ± 8.3
Dioxane 15%	505.4 ± 22.8	GLC 8	94.8 ± 3.1	81.0°C	31.4 ± 0.7
Dioxane 30%	509.7 ± 47.3	GLC 9	98.4 ± 10.6	90.2°C	6.4 ± 1.3
DMSO 15%	263.7 ± 10.1	GLC 10	99.0 ± 5.3
DMSO 30%	770.1 ± 41.7	GLC 11	91.5 ± 6.3

All measurements were performed using the p NPP method. Standard errors of the mean values are shown. ^a The enzyme was incubated for 48 h at 4°C in this assay. ^b Reference compound/condition set as 100% activity. ^c LipC12 was incubated for 24 h at 4°C in each pH buffer. ^d The enzyme was incubated for 24 h at 4°C in this assay. ^e The enzyme was incubated for 1 h at each temperature.

ISSN: 1475-2859