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Figure 1 | Microbial Cell Factories

Figure 1

From: Identification and characterization of a new true lipase isolated through metagenomic approach

Figure 1

Isolation, purification and identification of LipC12. (a) Identification of a lipolytic clone that formed a hydrolysis halo in the tributyrin plate assay. (b) Hydrolysis halos formed by lipase-producing clones in the triolein plate assay. FosC12, FosE6 and FosH10 showed the largest hydrolysis halos and were chosen for sequencing. (c) SDS-PAGE of fractions of His-tagged LipC12 eluted from the affinity chromatography column. (d) SDS-PAGE and zymogram analyses of LipC12. The lanes correspond to molecular marker (MM), coomassie blue staining (CB) and lipolytic activity of LipC12 on the SDS-PAGE gel, using tricaprylin (TC) and tributyrin (TB) as substrates.

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