Figure 5
From: Optimizing HIV-1 protease production in Escherichia coli as fusion protein
Maturation of HIV-1Pr chimeric proteins by specific protease cleavage and isolation of HIV-1Pr. A) SDS-PAGE Analysis of enterokinase maturation of DsbA:HIV-1Pr precursor. B) GSTrap on-column cleavage of GST:HIVPr-His by PreScission protease for 24 hours at 5°C. Left: elution profile. Peak 1 was eluted in PreScission buffer, after protease cleavage; peak 2 was eluted with 50 mM TrisHCl pH 8.0, 15 mM reduced L-glutathione. Right: SDS-PAGE analysis of eluted fractions. C) Far-UV CD spectrum of purified mature HIV-1Pr (0.1 mg/mL) in storage buffer.