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Table 1 Expression summary and purification yields for cultures grown at 18°C

From: Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield

Vector Expressed protein Cellular location C41(DE3) E.coli isolated U24 (mg/L) Origami 2 E.coli isolated U24 (mg/L) Shuffle E.coli isolated U24 (mg/L)
pMAL-c2x MBP-6 × His-U24 Cytoplasm N/A 1.84 ± 0.31 1.60 ± 0.24
pMAL-p2x MBP-6 × His-U24 Periplasm 0.46 ± 0.27 2.81 ± 0.32 (M9) N/A N/A
  1. Results are based on the intensity of expected bands observed by SDS-PAGE and by the BCA assay. U24 was purified as described from the indicated strains grown in 4 × 1 L of LB (or M9), induced at 18°C. N/A: Not applicable. Concentration is the mean ± s.d. of n = 4-6 independent assay measurements.