Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield

Table 1 Expression summary and purification yields for cultures grown at 18°C

Vector	Expressed protein	Cellular location	C41(DE3) E.coli isolated U24 (mg/L)	Origami 2 E.coli isolated U24 (mg/L)	Shuffle E.coli isolated U24 (mg/L)
pMAL-c2x	MBP-6 × His-U24	Cytoplasm	N/A	1.84 ± 0.31	1.60 ± 0.24
pMAL-p2x	MBP-6 × His-U24	Periplasm	0.46 ± 0.27 2.81 ± 0.32 (M9)	N/A	N/A

Results are based on the intensity of expected bands observed by SDS-PAGE and by the BCA assay. U24 was purified as described from the indicated strains grown in 4 × 1 L of LB (or M9), induced at 18°C. N/A: Not applicable. Concentration is the mean ± s.d. of n = 4-6 independent assay measurements.

ISSN: 1475-2859