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Figure 4 | Microbial Cell Factories

Figure 4

From: Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield

Figure 4

Far-UV CD spectra of U24 obtained from the different cell strains used in this study. U24 protein that had been isolated from various E. coli cell strains was reconstituted in 10 mM Tris·HCl, 10 mM NaCl, 10 mM SDS, pH 7.5 and far-UV CD was run in the presence and absence of tris(2-carboxyethyl)phosphine (TCEP) reducing agent. Concentration of protein in the samples were determined by BCA assay to be [U24]C41(DE3) = 0.106 ± 0.004 mg/ml, [U24]Origami = 0.093 ± 0.006 mg/ml, and [U24]SHuffle = 0.118 ± 0.004 mg/ml, where the standard deviation is based on four independent assay measurements.

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