Figure 3

HPLC and TLC analyses of the xylose mother liquor treated with C. maltosa and B. subtilis strain BSxyl. A, xylose mother liquor containing glucose (showed by solid line), xylose (8.407 min), galactose (showed by dotted line), L-arabinose (10.307 min), and unknown sugars (6.665 min and 12.390 min); B, xylose mother liquor treated with C. maltosa for 12 h (glucose is depleted); C, B. subtilis strain BSxyl was inoculated in xylose mother liquor pretreated with C. maltosa for 60 h. L-arabinose as well as some galactose and unknown sugars (6.682 min and 12.348 min) were depleted. D, TLC analysis of the B. subtilis strain BSxyl final fermentation medium (C) to detect whether galactose is still present. Lane 1, xylose mother liquor; lane 2, B. subtilis strain BSxyl final fermentation medium (C); lanes 3 to5, xylose, galactose, and L-arabinose standards, respectively.