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Table 1 Gene specific primers used for amplification of chromosomal DNA of Bacillus subtilis SK320

From: Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release

Gene Primer Sequence 5'-3' Recombinants (designation)
sfp 5'-CGTTCGCTCAGTCATAAGCA-3' BioS a
  5'-CCTGTATGCACACCCATCTG-3'  
sfp0 5'-CTAGAATTCAGATTTACGGAATTTATATG-3' BioS b
  5'-GGGGAATTCAGGGTGTGCGGCGCATAC--3'  
srfA 5'-TCCGTTTTTCCTTGTTCACC-3' BioS c
  5'-TCTTTCTGCCACTGCATCAC-3'