Table 1 Gene specific primers used for amplification of chromosomal DNA of Bacillus subtilis SK320
Gene | Primer Sequence 5'-3' | Recombinants (designation) |
|---|---|---|
sfp | 5'-CGTTCGCTCAGTCATAAGCA-3' | BioS a |
| Â | 5'-CCTGTATGCACACCCATCTG-3' | Â |
sfp0 | 5'-CTAGAATTCAGATTTACGGAATTTATATG-3' | BioS b |
| Â | 5'-GGGGAATTCAGGGTGTGCGGCGCATAC--3' | Â |
srfA | 5'-TCCGTTTTTCCTTGTTCACC-3' | BioS c |
| Â | 5'-TCTTTCTGCCACTGCATCAC-3' | Â |