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Table 1 Gene specific primers used for amplification of chromosomal DNA of Bacillus subtilis SK320
| Gene | Primer Sequence 5'-3' | Recombinants (designation) |
|---|---|---|
| sfp | 5'-CGTTCGCTCAGTCATAAGCA-3' | BioS a |
| 5'-CCTGTATGCACACCCATCTG-3' | ||
| sfp0 | 5'-CTAGAATTCAGATTTACGGAATTTATATG-3' | BioS b |
| 5'-GGGGAATTCAGGGTGTGCGGCGCATAC--3' | ||
| srfA | 5'-TCCGTTTTTCCTTGTTCACC-3' | BioS c |
| 5'-TCTTTCTGCCACTGCATCAC-3' |
