Skip to main content
Figure 4 | Microbial Cell Factories

Figure 4

From: Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the Hepatitis B surface antigen

Figure 4

Immunogold labeling and close-up views of P. pastoris GS115 producing HBsAg. The antigen was labeled with rabbit antibodies specific for HBsAg and is shown by dark Protein A-gold conjugates, localized in the brighter 'irregular cloud-shaped area' (*). (A-D) Representative images of cells grown on methanol as described in the Materials and Methods section. The insert in B represents a close-up view of the boxed area in B with the white arrows pointing to the cytoplasmic side of the membrane surrounding the 'irregular cloud-shaped area' (*). C1 and C2 represent sequential enlargements of the corresponding boxed area in C. Black arrows (C2) point to the lamellar striation within the labeled 'irregular cloud-shaped area' (*). Black and white arrowheads in D1 point to the cytosolic and nucleic side of the nuclear double membrane, respectively. Abbreviations as specified in Figure 1

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us