Figure 2

³⁴S labeling in chemostat culture. (A + C) and (B + D) respectively derive from two independent, replicate fermentations. In (A) and (B) the decrease of the ³²S content of intra- and extracellular Fab3H6 during continuous ³⁴S labeling in chemostat cultivation is shown. Additionally, in (A) the ³²S content of small compounds, mainly sulfate, in the media was measured (open circles). The linear regression analysis of the normalized ³²S contents of the intracellular Fab3H6 (equation 8) is presented in (C) and (D). The dashed lines illustrate the 95% confidence intervals. The slope represents the sum of the time constants of protein secretion, degradation and dilution by growth.