Figure 1From: Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedureImmunoprecipitation of Fab3H6. Immunoprecipitation of extracellular and intracellular Fab3H6 during 34S-labeling. Lanes 2-6: consecutive extracellular samples, lanes 7-11: consecutive intracellular samples. Immunoprecipitates were separated by non-reduced SDS-PAGE and silver stained. Dimers and monomers of the Fab fragment are indicated by arrows. The protein ladder (Page Ruler, Invitrogen) is seen in lane 1.Back to article page