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Table 4 β-Galactosidase activity of L. plantarum WCFS1 harboring pEH9R, pEH9A and pEH9A2a

From: Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

Volumetric activity (kU/L)

   

Time (h)

WCFSI + pEH9A2

WCFS1 + pEH9A

WCFS1 + pEH9R

Ratio (pEH9A2/pEH9A)

6

0.23 ± 0.00

0.17 ± 0.00

2.45 ± 0.10

1.43

8

1.34 ± 0.05

1.09 ± 0.08

12.0 ± 0.4

1.23

12

3.00 ± 0.12

2.40 ± 0.19

76.3 ± 4.1

1.25

24

3.17 ± 0.15

2.69 ± 0.31

101 ± 6

1.19

Specific activity (U/mg)

   

Time (h)

WCFSI + pEH9A2

WCFS1 + pEH9A

WCFS1 + pEH9R

Ratio (pEH9A2/pEH9A)

6

1.15 ± 0.04

0.85 ± 0.08

16.1 ± 0.1

1.36

8

2.83 ± 0.08

2.60 ± 0.07

39.0 ± 3.4

1.09

12

3.18 ± 0.10

2.67 ± 0.13

95.3 ± 0.2

1.19

24

3.31 ± 0.41

2.85 ± 0.33

97.1 ± 5.7

1.16

  1. a The method used for cell disruption used in this study differed from the method used to produce Fig. 1 (see Materials and Methods). This explains why the absolute enzyme activity values vary between the two experiments.