Skip to main content

Table 4 β-Galactosidase activity of L. plantarum WCFS1 harboring pEH9R, pEH9A and pEH9A2a

From: Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

Volumetric activity (kU/L)    
Time (h) WCFSI + pEH9A2 WCFS1 + pEH9A WCFS1 + pEH9R Ratio (pEH9A2/pEH9A)
6 0.23 ± 0.00 0.17 ± 0.00 2.45 ± 0.10 1.43
8 1.34 ± 0.05 1.09 ± 0.08 12.0 ± 0.4 1.23
12 3.00 ± 0.12 2.40 ± 0.19 76.3 ± 4.1 1.25
24 3.17 ± 0.15 2.69 ± 0.31 101 ± 6 1.19
Specific activity (U/mg)    
Time (h) WCFSI + pEH9A2 WCFS1 + pEH9A WCFS1 + pEH9R Ratio (pEH9A2/pEH9A)
6 1.15 ± 0.04 0.85 ± 0.08 16.1 ± 0.1 1.36
8 2.83 ± 0.08 2.60 ± 0.07 39.0 ± 3.4 1.09
12 3.18 ± 0.10 2.67 ± 0.13 95.3 ± 0.2 1.19
24 3.31 ± 0.41 2.85 ± 0.33 97.1 ± 5.7 1.16
  1. a The method used for cell disruption used in this study differed from the method used to produce Fig. 1 (see Materials and Methods). This explains why the absolute enzyme activity values vary between the two experiments.