Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. β-galactosidases in Lactobacillus plantarum

Table 1 Oligonucleotide primers used in this study

Primer	Sequence 5' - 3'^a	T_a^b(°C)	Concentration^c(nM)	Product size (bp)	Tm product (°C)
16S_f	TGATCCTGGCTCAGGACGAA	60	250	81	81
16S_r	TGCAAGCACCAATCAATACCA		250
Ery^R_f	CCGTGCGTCTGACATCTAT	60	250	108	79
Ery^R_r	TGCTGAATCGAGACTTGAGTG		250
LacReu_f	CCA GAT TCC GTG GTA TTA CCT TTG TG	60	250	154	80
LacReu_r	TAC TACT ACG TCA CGC CAT TGA GGA AC		500
LacAci_f	TCTAGTTCACTACGAAGGTGTCG	60	500	154	76.5
LacAci_r	GTCATGCATGTATTCACACTCC		500
SppKR_f	CAAGCCGTTCAAGAAACCGAT	60	250	144	78.5
SppKR_r	AGCGCCTTTCGTTGAATAGCC		500
11n15_f	GATGACCCG GAAATTTTTCGC GTCAATCAATTGCCAGC
11n15_r	GACGCG AAAAATTTCCGG GTCATC

ISSN: 1475-2859