Figure 3
From: A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification
HPLC analysis of enzymatic reaction products. Crude cell free extracts were used for analysis of various enzyme activities. The reactions were allowed to run overnight and the solutions were extracted with phenol to remove proteins. Aliquots (40 μl) from the various reactions were applied to HPLC column: A1, 1% (w/v) L-arabinose standard, no enzymatic reaction; A2-A4, 1% L-arabinose plus crude extracts of Y161 grown with galactose, xylose or xylitol as a carbon source, respectively. B1, 1% (w/v) L-arabitol standard, no enzyme; B2-B4, L-arabitol plus crude extracts of Y161 grown with galactose, xylose or xylitol as a carbon source, respectively. C1, 1% (v/v) L-xylulose standard; C2-C4, L-xylulose plus crude extracts of Y161 grown with galactose, xylose or xylitol as a carbon source, respectively.