Table 1 Protein quantification and activity assaysa
From: Streamlined protein expression and purification using cleavable self-aggregating tags
Product protein (molecular weight) | Aggregatesb (μg/mg wet cell pellet) | Quantity of purified proteinb (μg/mg wet cell pellet) | Specific activity (units/mg) | Specific activity reported in the literature (units/mg) | Cleavage efficiencyc | Percent recovery (mass)d |
|---|---|---|---|---|---|---|
From 18A fusions | ||||||
LipA (21 kDa) | 34.1 ± 4.1 | 10.4 ± 0.3 | 72.8 ± 3.9 | (78 ± 2)% | (68 ± 7)% | |
AMA (49 kDa) | 19.1 ± 1.4 | 7.9 ± 1.0 | 2.3 ± 0.5 | (80 ± 1)% | (62 ± 3)% | |
XynB (61 kDa) | 18.1 ± 2.0 | 1.6 ± 0.2 | 2.6 ± 0.3 | (69 ± 3)% | (14 ± 1)% | |
From ELK16 fusions | ||||||
LipA | 31.0 ± 4.1 | 8.3 ± 0.7 | 133.4 ± 2.0 | 120 (ref. [21]) | (73 ± 1)% | (59 ± 4)% |
AMA | 23.2 ± 2.9 | 4.0 ± 0.1 | 1.8 ± 0.3 | 1.9-2.5 (ref. [14]) | (65 ± 2)% | (27 ± 3)% |
XynB | 17.6 ± 0.5 | 2.9 ± 0.1 | 1.5 ± 0.1 | 0.072-25.2e (ref. [13]) | (57 ± 2)% | (23 ± 1)% |