Figure 3
From: Streamlined protein expression and purification using cleavable self-aggregating tags
Fusion protein (target protein-intein-18A/ELK16) expression and cleavage. (A) LipA-I-18A. (B) LipA-I-ELK16. (C) AMA-I-18A. (D) AMA-I-ELK16. (E) XynB-I-18A. (F) XynB-I-ELK16. For (A-F): lane 1, insoluble fraction of cell lysate; lane 2, insoluble fraction of cleaved fusion protein; lane3, soluble fraction of cleaved fusion protein; lane 4, 5 and 6, bovine serum albumin (BSA) standards, at 6, 3 and 0.75 μg/lane, respectively.