Map and Southern analysis of Rut-C30 and putative Δ pgi1 mutants. (A) Rut-C30 genomic pgi1. (B) Correct insertion of the deletion cassette in the Δpgi1 mutants. Probes used for Southern experiments are indicated as bars that are located in the corresponding region of the DNA where they hybridize. (C) Southern analysis. Genomic DNA of strain RutC-30 and 19 putative transformants were digested with Pst I. Filter was probed with a 0.6-kb Pst I fragment comprising part of the coding region and part of the 5' UTR of the pgi gene.