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Table 1 Yeast strains used in this study

From: Improving yeast strains using recyclable integration cassettes, for the production of plant terpenoids

Strain

Genotype

Plasmid description

Source

BY4741-01

Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0.

pJG4-6/SfCinS1 2μ TRP1 PGal1-HA-SfCinS1

This study

BY4741-02

Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

BY4741-04

Mat a, his3 Δ1, leu2 Δ0, met15 Δ0, ura3 Δ0.

pYESmyc/P330 2μ URA3 PGal1-myc-P330

This study

EGY48

Mat α, ura3, trp1, his3, 6xLexA operators::LEU2. Derivative of the U457.

 

Erica Golemis

EGY48-01

Mat α, ura3, trp1, his3, 6xLexA operators::LEU2. Derivative of the U457.

pYES/SfCinS1-LexA 2μ URA3 PGal1-SfCinS1-LexA

This study

EGY48-02

Mat α, ura3, trp1, his3, 6xLexA operators::LEU2. Derivative of the U457.

pYES-LexA 2μ URA3 PGal1-LexA

This study

EG60

Mat α, ura3, trp1, his3, leu2.

 

Erica Golemis

EG60-01

Mat α, ura3, trp1, his3, leu2

pJG4-6/SfCinS1 2μ TRP1 PGal1-HA-SfCinS1

This study

EG60-02

Mat α, ura3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

EG60-03

Mat α, ura3, trp1, his3, leu2

pYESmyc/P330 2μ URA3 PGal1-myc-P330

This study

EG60-04

Mat α, ura3, trp1, his3, leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

EG60-05

Mat α, ura3, trp1, his3 leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYES 2μ URA3 PGal1

This study

EG60-06

Mat α, ura3, trp1, his3 leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYES/HSP90 2μ URA3 PGal1-HSP90

This study

EG60-07

Mat α, ura3, trp1, his3 leu2

pJG4-4 2μ TRP1 PGal1 - empty vector

 

EG61

Mat a, ura3, trp1, his3 leu2

 

This study

KSY10

Mat a, PGal1-HMG2 (K6R)::URA3, trp1, his3, leu2

 

This study

AM62

Mat α, Δerg6:: HIS5, ura3, trp1, his3, leu2 Derivative of EG60.

 

This study

AM63

Mat α, PGal1-HMG2 (K6R)::URA3, trp1, his3, leu2.

 

This study

AM63-01

Mat α, PGal1-HMG2 (K6R)::URA3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM63-02

Mat α, PGal1-HMG2 (K6R)::URA3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYX143/GDS 2μ LEU2 PTPI1-GDS

This study

AM64

Mat α, URA3-hisG-PGal1-HMG2 (K6R)::HO, trp1, his3, leu2, Δerg6::HIS5. Derivative of AM63.

 

This study

AM65

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2. Derivative of AM64.

 

This study

AM65-01

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1,

This study

AM65-02

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3,leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

 

AM66

Mat α/a, PGal1-HMG2 (K6R) X2::HO, ura3, trp1, his3, leu2. Derivative of AM63 and KSY10 back-crossed 4 times.

 

This study

AM66-01

Mat α/a, PGal1-HMG2 (K6R) x2::HO, ura3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM66-02

Mat α/a, PGal1-HMG2 (K6R) x2::HO, ura3, trp1, his3, leu2

pYESmyc/P330 2μ URA3 PGal1-myc-P330

This study

AM66-03

Mat α/a, PGal1-HMG2 -(K6R) x2::HO, ura3, trp1, his3, leu2.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

AM67

Mat α/a, PGal1-HMG2 (K6R) x2, PGal1-HA-ERG20-URA3, trp1, his3, leu2. Derivative of AM66.

 

This study

AM67-01

Mat α/a, PGal1-HMG2 (K6R)x2, PGal1-HA-g-URA3, trp1, his3, leu2..

pB227/GAL-cre, CEN LEU2

This study

AM67-02

Mat α/a, PGal1 -HMG2 (K6R) x2, PGal1-HA-ERG20-URA3, trp1, his3, leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM68

Mat α/a, PGal1-HMG2 -(K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3. Derivative of AM67.

 

This study

AM68-01

Mat α/a, PGal1-HMG2 (K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM68-02

Mat α/a, PGal1-HMG2 -(K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2

pYESmyc/P330 2μ URA3 PGal1-myc-P330

This study

AM68-03

Mat α/a, PGal1-HMG2 (K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

AM68-04

Mat α/a, PGal1-HMG2 (K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2

pYESmyc/P330 2μ URA3 PGal1-myc-P330, pYIC1/IDI1 2μ LEU2 PGal1-IDI1

This study

AM69

Mat α/a, PGal1-HMG2 -(K6R)x2, PGal1-ERG20-URA3, trp1, his3, leu2, Δerg9:: HIS5. Derivative of AM67.

 

This study

AM70

Mat α/a, PGal1-HMG2 -(K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2, Δerg9:: HIS5. Derivative of AM68.

 

This study

AM70-01

Mat α/a, PGal1-HMG2 (K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2, Δerg9:: HIS5.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM70-02

Mat α/a, PGal1-HMG2 -(K6R) x2, PGal1-HA-ERG20, ura3, trp1, his3, leu2, Δerg9:: HIS5.

pYESmyc/P330 2μ URA3 PGAL1-myc-P330

This study

AM70-03

Mat α/a, PGal1-HMG2 (K6R) x2,PGal1-HA-ERG20, ura3, trp1, his3, leu2, Δerg9:: HIS5.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

AM70-04

Mat α/a, PGal1-HMG2 (K6R) x2,PGal1-HA-ERG20, ura3, trp1, his3, leu2, Δerg9:: HIS5.

pYESmyc/P330 2μ URA3 PGAL1-myc-P330, pYIC1/IDI1 2μ LEU2 PGal1-IDI1

This study

AM74

Mat α/a, PGal1-HMG2 (K6R)x2::HO, PGal1-HA-ERG20, PAdh1-myc::tHMG1, URA3, trp1, his3, leu2. Derivative of AM68.

 

This study

AM75

Mat α/a, PGal1-HMG2 (K6R) x2::HO, PGal1-HA-ERG20, PAdh1-myc::tHMG1, URA3, trp1, his3, leu2, Δerg9::HIS5. Derivative of AM70.

 

This study

AM76

Mat α/a, PGal1-HMG2 (K6R) x2::HO, ura3, trp1, his3, leu2, Δerg9:: HIS5. Derivative of AM66.

 

This study

AM76-01

Mat α/a, PGal1-HMG2 (K6R) x2::HO, ura3, trp1, his3, leu2, Δerg9:: HIS5.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

AM77

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1-HIS5. Derivative of AM65.

 

This study

AM77-01

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1-HIS5.

pB227/GAL-cre, CEN LEU2

This study

AM77-02

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGAL1-IDI1-HIS5.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM78

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1. Derivative of AM77.

 

This study

AM78-01

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1

This study

AM78-02

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP 1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1

This study

AM78-03

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1, pYIC1 2μ LEU2 PGal1

This study

AM78-03

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc 2μ URA3 PGal1-myc, pYIC1/IDI1 2μ LEU2 PGal1-HA-IDI1

This study

AM78-04

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYIC1/IDI1 2μ LEU2 PGal1-HA-IDI1

This study

AM78-05

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1, pYIC1/IDI1 2μ LEU2 PGal1-HA-IDI1

This study

AM78-06

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc 2μ URA3 PGal1-myc-, pYIC1/HMG2(K6R) 2μ LEU 2 PGal1-HA-HMG2 (K6R)

This study

AM78-07

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYESmyc/IDI1 2μ URA3 PGal1-myc-IDI1, pYIC1/HMG2(K6R) 2μ LEU 2 PGal1-HA-HMG2 (K6R)

This study

AM78-08

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYES 2μ URA3 PGal1

This study

AM78-09

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYES/HSP90 2μ URA3 PGal1-HSP90

This study

AM78-10

Mat α, PGal1-HMG2 (K6R), ura3, trp1, his3, leu2:: PGal1-IDI1.

pJG4-4/SfCinS1 2μ TRP1 PGal1-SfCinS1, pYIC1/HMG2(K6R) 2μ LEU2 PGal1-HA-HMG2 (K6R)

This study

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