Figure 8From: Improving yeast strains using recyclable integration cassettes, for the production of plant terpenoidsThe putative interacting HSP90 synergizes with SfCinS1 to increase cineole production. (A) EGY48 yeast reporter cells carrying the SfCinS1-LexA (top row) or the LexA plasmid (bottom row) with the five (C2, C4, D1, D4, D6) library cDNAs were replica plated on glucose/CM-his, trp, leu and galactose-raffinose/CM-his, trp, leu agar media. Growth is observed only for the SfCinS1-LexA fusion in the galactose based media, where proteins are expressed, confirming the specificity of interactions. (B) EG60-05 cells and AM78-08 cells carrying SfCinS1 with empty vector (light grey) and EG60-05 and AM78-09 cells carrying SfCinS1 with HSP90 (dark grey), were tested for cineole production. The presence of HSP90 enhances cineole yield for both strains.Back to article page