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Figure 8 | Microbial Cell Factories

Figure 8

From: Improving yeast strains using recyclable integration cassettes, for the production of plant terpenoids

Figure 8

The putative interacting HSP90 synergizes with SfCinS1 to increase cineole production. (A) EGY48 yeast reporter cells carrying the SfCinS1-LexA (top row) or the LexA plasmid (bottom row) with the five (C2, C4, D1, D4, D6) library cDNAs were replica plated on glucose/CM-his, trp, leu and galactose-raffinose/CM-his, trp, leu agar media. Growth is observed only for the SfCinS1-LexA fusion in the galactose based media, where proteins are expressed, confirming the specificity of interactions. (B) EG60-05 cells and AM78-08 cells carrying SfCinS1 with empty vector (light grey) and EG60-05 and AM78-09 cells carrying SfCinS1 with HSP90 (dark grey), were tested for cineole production. The presence of HSP90 enhances cineole yield for both strains.

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