Figure 5
High levels of IDI1 lead to high monoterpene production. (A) Co-expression from a plasmid of the yeast IDI1 gene in EG60-04, AM65-02, AM68-03, AM70-03, AM76-01 cells (dark bars) leads to substantial increase in cineole yields in all strains tested vs. their corresponding non expressing strains (light grey bars); (B) Map of COD4 cassette which can accept cDNAs under the control of the galactose promoter; (C) Map of COD40 cassette which integrates an extra copy of the IDI1 gene into the chromosome, under the control of the galactose promoter. Subsequent to integration, the loxP-his5-loxP part of the cassette is removed by excision from the Cre recombinase; (D) Cineole production in AM78-01 and AM78-02 cells, which have a copy of the PGal1-IDI1-ts in the leu2 locus.