Figure 3

Integration of HMG2(K6R) variant leads to cineole production increase. (A) Map of the homologous recombination cassette designed to integrate a stabilized copy of HMG2(K6R) into the HO locus under the control of the galactose promoter. The hisG-URA3-hisG part of the cassette is subsequently excised from the genome by plating in FOA plates; (B) Cineole production in AM63 and AM65 cells; (C) Cineole yields in AM63 cells co-expressing the P. abies geranyl pyrophosphate synthase (GDS). Exogenous GPP was also added to assess the level of saturation of the system.