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Table 3 Fermentation characteristics of the wild type E. coli and its phoB mutant in the aerobic chemostat culture under different nitrogen and phosphate concentrations at the dilution rate of 0.2 h-1 at pH 7.0.

From: Metabolic regulation of Escherichia coli and its phoB and phoR genes knockout mutants under phosphate and nitrogen limitations as well as at acidic condition

Fermentation parameters

 

N and P-rich condition (100%)

N -limited (20%) and P-rich condition

N-limited (20%) and lower P concentration (55%)

Biomass concentration (g/l)

Wild

3.86 ± 0.03

1.753 ± 0.005

1.68 ± 0.01

 

ΔphoB

3.44 ± 0.04

1.703 ± 0.005

1.59 ± 0.01

Glucose concentration (g/l)

Wild

0.660 ± 0.004

5.39 ± 0.01

4.10 ± 0.01

 

ΔphoB

1.59 ± 0.29

2.890 ± 0.006

4.81 ± 0.01

Acetate concentration (g/l)

Wild

0.046 ± 0.002

0.486 ± 0.002

0.502 ± 0.004

 

ΔphoB

0.255 ± 0.130

0.475 ± 0.004

0.495 ± 0.010

Specific glucose uptake rate (mmol/gDCW/h)

Wild

2.69 ± 0.05

2.92 ± 0.01

3.90 ± 0.01

 

ΔphoB

2.72 ± 0.09

4.64 ± 0.01

3.63 ± 0.02

Specific acetate production rate (mmol/gDCW/h)

Wild

0.040 ± 0.002

0.923 ± 0.010

0.995 ± 0.010

 

ΔphoB

0.247 ± 0.080

0.928 ± 0.010

1.037 ± 0.020

  1. Note: The standard deviation was obtained by triplicate measurements.