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Table 2 Fermentation characteristics of the wild type E. coli and its phoB mutant in the aerobic chemostat culture under two phosphate concentrations (100% and 55%) and two pH values (7.0 and 6.0) at the dilution rate of 0.2 h-1.

From: Metabolic regulation of Escherichia coli and its phoB and phoR genes knockout mutants under phosphate and nitrogen limitations as well as at acidic condition

Fermentation parameters

P-rich condition (100%)

Lower P concentration (55%)

  

pH 7.0

pH 6.0

pH 6.0

Biomass concentration (g/l)

Wild

3.86 ± 0.03

3.680 ± 0.002

1.960 ± 0.001

 

ΔphoB

3.44 ± 0.04

_

3.040 ± 0.001

Glucose concentration (g/l)

Wild

0.660 ± 0.004

1.053 ± 0.010

0.677 ± 0.050

 

ΔphoB

1.59 ± 0.29

_

1.145 ± 0.020

Acetate concentration (g/l)

Wild

0.046 ± 0.002

0.468 ± 0.003

0.519 ± 0.001

 

ΔphoB

0.255 ± 0.130

_

0.510 ± 0.007

Specific glucose uptake rate (mmol/gDCW/h)

Wild

2.69 ± 0.05

2.700 ± 0.001

5.285 ± 0.031

 

ΔphoB

2.72 ± 0.09

_

3.24 ± 0.01

Specific acetate production rate (mmol/gDCW/h)

Wild

0.040 ± 0.002

0.424 ± 0.003

0.882 ± 0.001

 

ΔphoB

0.247 ± 0.080

_

0.559 ± 0.007

  1. Note: " - " indicates that no data was collected for this condition. The standard deviation was obtained by triplicate measurements.