Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b

Table 4 Lay out of the experiments conducted to study the effect of PTM1 components on protease activity.

Exp No	h3	co	zn	fe	k	mn	cu	na
1	p	p	p	p	p	p	a	a
2	a	p	p	p	p	p	p	p
3	p	a	p	p	p	p	p	p
4	a	a	p	p	p	p	a	a
5	p	p	a	p	p	p	p	a
6	a	p	a	p	p	p	a	p
7	p	a	a	p	p	p	a	p
8	a	a	a	p	p	p	p	a
9	p	p	p	a	p	p	p	a
10	a	p	p	a	p	p	a	p
11	p	a	p	a	p	p	a	p
12	a	a	p	a	p	p	p	a
13	p	p	a	a	p	p	a	a
14	a	p	a	a	p	p	p	p
15	p	a	a	a	p	p	p	p
16	a	a	a	a	p	p	a	a
17	p	p	p	p	a	p	a	p
18	a	p	p	p	a	p	p	a
19	p	a	p	p	a	p	p	a
20	a	a	p	p	a	p	a	p
21	p	p	a	p	a	p	p	p
22	a	p	a	p	a	p	a	a
23	p	a	a	p	a	p	a	a
24	a	a	a	p	a	p	p	p
25	p	p	p	a	a	p	p	p
26	a	p	p	a	a	p	a	a
27	p	a	p	a	a	p	a	a
28	a	a	p	a	a	p	p	p
29	p	p	a	a	a	p	a	p
30	a	p	a	a	a	p	p	a
31	p	a	a	a	a	p	p	a
32	a	a	a	a	a	p	a	p
33	p	p	p	p	p	a	a	p
34	a	p	p	p	p	a	p	a
35	p	a	p	p	p	a	p	a
36	a	a	p	p	p	a	a	p
37	p	p	a	p	p	a	p	p
38	a	p	a	p	p	a	a	a
39	p	a	a	p	p	a	a	a
40	a	a	a	p	p	a	p	p
41	p	p	p	a	p	a	p	p
42	a	p	p	a	p	a	a	a
43	p	a	p	a	p	a	a	a
44	a	a	p	a	p	a	p	p
45	p	p	a	a	p	a	a	p
46	a	p	a	a	p	a	p	a
47	p	a	a	a	p	a	p	a
48	a	a	a	a	p	a	a	p
49	p	p	p	p	a	a	a	a
50	a	p	p	p	a	a	p	p
51	p	a	p	p	a	a	p	p
52	a	a	p	p	a	a	a	a
53	p	p	a	p	a	a	p	a
54	a	p	a	p	a	a	a	p
55	p	a	a	p	a	a	a	p
56	a	a	a	p	a	a	p	a
57	p	p	p	a	a	a	p	a
58	a	p	p	a	a	a	a	p
59	p	a	p	a	a	a	a	p
60	a	a	p	a	a	a	p	a
61	p	p	a	a	a	a	a	a
62	a	p	a	a	a	a	p	p
63	p	a	a	a	a	a	p	p
64	a	a	a	a	a	a	a	a
65	p	p	p	p	p	p	p	p
66	p	p	p	p	p	p	p	p
67	p	p	p	p	p	p	p	p

The experiments were designed by Modde 6.0 software.
Abbreviations: h3 (H₃BO₃); cu (CuSO₄.5H₂O); k (KI); mn (MnSO₄.2H₂O); fe (FeSO₄.7H₂O); na (Na₂MoO₄.2H₂O); co (CoCl₂.6H₂O) and zn (ZnCl₂). (p) means presence and (a) stands for absence.

ISSN: 1475-2859

Exp No	h3	co	zn	fe	k	mn	cu	na
1	p	p	p	p	p	p	a	a
2	a	p	p	p	p	p	p	p
3	p	a	p	p	p	p	p	p
4	a	a	p	p	p	p	a	a
5	p	p	a	p	p	p	p	a
6	a	p	a	p	p	p	a	p
7	p	a	a	p	p	p	a	p
8	a	a	a	p	p	p	p	a
9	p	p	p	a	p	p	p	a
10	a	p	p	a	p	p	a	p
11	p	a	p	a	p	p	a	p
12	a	a	p	a	p	p	p	a
13	p	p	a	a	p	p	a	a
14	a	p	a	a	p	p	p	p
15	p	a	a	a	p	p	p	p
16	a	a	a	a	p	p	a	a
17	p	p	p	p	a	p	a	p
18	a	p	p	p	a	p	p	a
19	p	a	p	p	a	p	p	a
20	a	a	p	p	a	p	a	p
21	p	p	a	p	a	p	p	p
22	a	p	a	p	a	p	a	a
23	p	a	a	p	a	p	a	a
24	a	a	a	p	a	p	p	p
25	p	p	p	a	a	p	p	p
26	a	p	p	a	a	p	a	a
27	p	a	p	a	a	p	a	a
28	a	a	p	a	a	p	p	p
29	p	p	a	a	a	p	a	p
30	a	p	a	a	a	p	p	a
31	p	a	a	a	a	p	p	a
32	a	a	a	a	a	p	a	p
33	p	p	p	p	p	a	a	p
34	a	p	p	p	p	a	p	a
35	p	a	p	p	p	a	p	a
36	a	a	p	p	p	a	a	p
37	p	p	a	p	p	a	p	p
38	a	p	a	p	p	a	a	a
39	p	a	a	p	p	a	a	a
40	a	a	a	p	p	a	p	p
41	p	p	p	a	p	a	p	p
42	a	p	p	a	p	a	a	a
43	p	a	p	a	p	a	a	a
44	a	a	p	a	p	a	p	p
45	p	p	a	a	p	a	a	p
46	a	p	a	a	p	a	p	a
47	p	a	a	a	p	a	p	a
48	a	a	a	a	p	a	a	p
49	p	p	p	p	a	a	a	a
50	a	p	p	p	a	a	p	p
51	p	a	p	p	a	a	p	p
52	a	a	p	p	a	a	a	a
53	p	p	a	p	a	a	p	a
54	a	p	a	p	a	a	a	p
55	p	a	a	p	a	a	a	p
56	a	a	a	p	a	a	p	a
57	p	p	p	a	a	a	p	a
58	a	p	p	a	a	a	a	p
59	p	a	p	a	a	a	a	p
60	a	a	p	a	a	a	p	a
61	p	p	a	a	a	a	a	a
62	a	p	a	a	a	a	p	p
63	p	a	a	a	a	a	p	p
64	a	a	a	a	a	a	a	a
65	p	p	p	p	p	p	p	p
66	p	p	p	p	p	p	p	p
67	p	p	p	p	p	p	p	p

Exp No	h3	co	zn	fe	k	mn	cu	na
1	p	p	p	p	p	p	a	a
2	a	p	p	p	p	p	p	p
3	p	a	p	p	p	p	p	p
4	a	a	p	p	p	p	a	a
5	p	p	a	p	p	p	p	a
6	a	p	a	p	p	p	a	p
7	p	a	a	p	p	p	a	p
8	a	a	a	p	p	p	p	a
9	p	p	p	a	p	p	p	a
10	a	p	p	a	p	p	a	p
11	p	a	p	a	p	p	a	p
12	a	a	p	a	p	p	p	a
13	p	p	a	a	p	p	a	a
14	a	p	a	a	p	p	p	p
15	p	a	a	a	p	p	p	p
16	a	a	a	a	p	p	a	a
17	p	p	p	p	a	p	a	p
18	a	p	p	p	a	p	p	a
19	p	a	p	p	a	p	p	a
20	a	a	p	p	a	p	a	p
21	p	p	a	p	a	p	p	p
22	a	p	a	p	a	p	a	a
23	p	a	a	p	a	p	a	a
24	a	a	a	p	a	p	p	p
25	p	p	p	a	a	p	p	p
26	a	p	p	a	a	p	a	a
27	p	a	p	a	a	p	a	a
28	a	a	p	a	a	p	p	p
29	p	p	a	a	a	p	a	p
30	a	p	a	a	a	p	p	a
31	p	a	a	a	a	p	p	a
32	a	a	a	a	a	p	a	p
33	p	p	p	p	p	a	a	p
34	a	p	p	p	p	a	p	a
35	p	a	p	p	p	a	p	a
36	a	a	p	p	p	a	a	p
37	p	p	a	p	p	a	p	p
38	a	p	a	p	p	a	a	a
39	p	a	a	p	p	a	a	a
40	a	a	a	p	p	a	p	p
41	p	p	p	a	p	a	p	p
42	a	p	p	a	p	a	a	a
43	p	a	p	a	p	a	a	a
44	a	a	p	a	p	a	p	p
45	p	p	a	a	p	a	a	p
46	a	p	a	a	p	a	p	a
47	p	a	a	a	p	a	p	a
48	a	a	a	a	p	a	a	p
49	p	p	p	p	a	a	a	a
50	a	p	p	p	a	a	p	p
51	p	a	p	p	a	a	p	p
52	a	a	p	p	a	a	a	a
53	p	p	a	p	a	a	p	a
54	a	p	a	p	a	a	a	p
55	p	a	a	p	a	a	a	p
56	a	a	a	p	a	a	p	a
57	p	p	p	a	a	a	p	a
58	a	p	p	a	a	a	a	p
59	p	a	p	a	a	a	a	p
60	a	a	p	a	a	a	p	a
61	p	p	a	a	a	a	a	a
62	a	p	a	a	a	a	p	p
63	p	a	a	a	a	a	p	p
64	a	a	a	a	a	a	a	a
65	p	p	p	p	p	p	p	p
66	p	p	p	p	p	p	p	p
67	p	p	p	p	p	p	p	p

Exp No	h3	co	zn	fe	k	mn	cu	na
1	p	p	p	p	p	p	a	a
2	a	p	p	p	p	p	p	p
3	p	a	p	p	p	p	p	p
4	a	a	p	p	p	p	a	a
5	p	p	a	p	p	p	p	a
6	a	p	a	p	p	p	a	p
7	p	a	a	p	p	p	a	p
8	a	a	a	p	p	p	p	a
9	p	p	p	a	p	p	p	a
10	a	p	p	a	p	p	a	p
11	p	a	p	a	p	p	a	p
12	a	a	p	a	p	p	p	a
13	p	p	a	a	p	p	a	a
14	a	p	a	a	p	p	p	p
15	p	a	a	a	p	p	p	p
16	a	a	a	a	p	p	a	a
17	p	p	p	p	a	p	a	p
18	a	p	p	p	a	p	p	a
19	p	a	p	p	a	p	p	a
20	a	a	p	p	a	p	a	p
21	p	p	a	p	a	p	p	p
22	a	p	a	p	a	p	a	a
23	p	a	a	p	a	p	a	a
24	a	a	a	p	a	p	p	p
25	p	p	p	a	a	p	p	p
26	a	p	p	a	a	p	a	a
27	p	a	p	a	a	p	a	a
28	a	a	p	a	a	p	p	p
29	p	p	a	a	a	p	a	p
30	a	p	a	a	a	p	p	a
31	p	a	a	a	a	p	p	a
32	a	a	a	a	a	p	a	p
33	p	p	p	p	p	a	a	p
34	a	p	p	p	p	a	p	a
35	p	a	p	p	p	a	p	a
36	a	a	p	p	p	a	a	p
37	p	p	a	p	p	a	p	p
38	a	p	a	p	p	a	a	a
39	p	a	a	p	p	a	a	a
40	a	a	a	p	p	a	p	p
41	p	p	p	a	p	a	p	p
42	a	p	p	a	p	a	a	a
43	p	a	p	a	p	a	a	a
44	a	a	p	a	p	a	p	p
45	p	p	a	a	p	a	a	p
46	a	p	a	a	p	a	p	a
47	p	a	a	a	p	a	p	a
48	a	a	a	a	p	a	a	p
49	p	p	p	p	a	a	a	a
50	a	p	p	p	a	a	p	p
51	p	a	p	p	a	a	p	p
52	a	a	p	p	a	a	a	a
53	p	p	a	p	a	a	p	a
54	a	p	a	p	a	a	a	p
55	p	a	a	p	a	a	a	p
56	a	a	a	p	a	a	p	a
57	p	p	p	a	a	a	p	a
58	a	p	p	a	a	a	a	p
59	p	a	p	a	a	a	a	p
60	a	a	p	a	a	a	p	a
61	p	p	a	a	a	a	a	a
62	a	p	a	a	a	a	p	p
63	p	a	a	a	a	a	p	p
64	a	a	a	a	a	a	a	a
65	p	p	p	p	p	p	p	p
66	p	p	p	p	p	p	p	p
67	p	p	p	p	p	p	p	p