Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b

Table 2 Composition of different growth media used in this study

Components (g/l)	SM1	SM2	SM3	SM4
K₂HPO₄	-	5.5	-	-
KH₂PO₄	1	15	2	-
K₂SO₄	-	-	-	18.2
MgSO₄.7H₂O	0.5	1	0.6	7.28
CaCl₂	0.1	-	5	-
NaCl	0.1	-	-	-
CaSO₄	-	-	-	0.93
KOH	-	-	-	4.4
(NH₄)₂SO₄	-	4	4.5	-
Urea	2	-	-	-
Glucose	20	20	20	20
H₃PO₄	-	-	-	26.7
Trace elements (mg/l)				^a
H₃BO₃	0.5	-	8	20
CuSO₄.5H₂O	0.04	-	100	6000
KI	0.1	-	0.9	80
FeCl₃.4H₂O	0.2	10	-	-
ZnSO₄.7H₂O	0.4	-	4000	-
MnSO₄. 2H₂O	0.4	-	6500	3000
EDTA	-	-	12540	-
NaH₂PO₄.H₂O	-	-	3000	-
FeSO₄.7H₂O	-	-	2500	6500
Na₂MoO₄.2H₂O	-	-	4	200
CoCl₂.6H₂O	-	-	7	500
NiSO₄.7H₂O	-	-	0.8	-
ZnCl₂	-	-	-	20000
Vitamins (μg/l)			^b
Biotin	8	8		80
Thiamin	200	100		-
Myo-inositol	4	2		-
pH	4.5	6	5.5	5

^a Concentration in the stock solution. 2 ml/l stock solution were added to SM4 medium
^b For SM3 medium, the vitamin solution is composed of 1 mg/l pantothenic acid, 1 mg/l nicotinic acid, 25 mg/l myo-inositol, 1 mg/l thiamin and 1 mg/l pyridoxol hydrochloride, 0.2 mg/l p-amino benzoic acid and 0.05 mg/l biotin. 1.5 ml of this solution were added to 1l of SM3 medium.

ISSN: 1475-2859