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Table 1 Growth characteristics of Y. lipolytica in the different media

From: Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b

 

SM1

SM2

SM4

CM

μa (h-1)

0.18

0.18

0.16

0. 22

Maximal Biomass (g/l)

24.01

17.72

14.26

40.1

IFN yield (μg/l)

14

3

120

125

Y( hIFN/X) (mg/g)

0.58 × 10-3

0.17 × 10-3

0.84 × 10-2

0.32 × 10-2

Residual oleic acid (g/l)

1.4

0.7

0.54

1.1

Final pH

3.64

2.72

3.18

7.78

Cell morphology

Mycelium

yeast cells

yeast cells

yeast cells+

Biological activity (UI/mg)

0.42 × 107

0.54 × 107

0.97 × 107

mycelium 1.03 × 107

  1. aμ; growth rate calculated from total biomass level (g/l).
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Microbial Cell Factories

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