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Figure 5 | Microbial Cell Factories

Figure 5

From: Design of an efficient medium for heterologous protein production in Yarrowia lipolytica: case of human interferon alpha 2b

Figure 5

Effect of PTM1 concentration. (I-a) effect of PTM1 concentration on JMY1852p strain growth, hIFN α2b production using 2 ml/l of PTM1 solution (I-b) and 5 ml/l of PTM1 solution (I-c). Cultures were carried in 5-l bioreactor with oleic acid as carbon and inducer source, data represent the mean of duplicate. Open triangle: 5 ml PTM1 addition; closed triangle: 2 ml PTM1 addition. (II-a) a 28 kDa protease detected in cultures supernatants (SN). Lane 1; molecular weight, lane 2; SN (5 ml/l PTM1), lane 3; SN (2 ml/l PTM1). Protease inhibition by pepstatin addition at 10 μM (II-b) and 5 ml/l of PTM1 addition (II-c).

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Microbial Cell Factories

ISSN: 1475-2859

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