Figure 9

Recombinant MuHN and MeH induce specific stress response in yeast cells. (A-I) 2D gel electrophoresis of yeast proteins. Samples were taken from yeast cells of S. cerevisiae AH22 strain, expressing MuHN (central panel; B-H) or MeH (right panel; C-I) and from control AH22 cells, transformed with empty vector pFGG3 (left panel; A-G). At the top (A-C) whole cell lysates, in the middle (D-F) fractions of proteins, soluble at high salt concentration, and in the bottom panel (G-I) proteins, insoluble under native conditions are shown. Solid arrows in B and C indicate proteins, identified by MS directly from whole cell lysates, whereas dotted arrows point to the proteins, identified from soluble and insoluble fractions. Numbers of identified protein spots correspond to those, given in Table 1. Protein molecular mass markers (120, 66, 45, 35, and 25 kDa) were run simultaneously in the left lane (M) of each 2D gel.