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Figure 8 | Microbial Cell Factories

Figure 8

From: Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae

Figure 8

Analysis of MeH expression in whole cell lysates from P. pastoris and S. cerevisiae. Whole cell lysates from P. pastoris (C, In, SP and SG) and S. cerevisiae (Sc) were analysed by Western blotting using anti-His antibody. M- marker, C- control, In- multicopy pPIC3.5K-MeH transformant for intracellular expression of full sequence MeH protein, SP- multicopy pPIC9K-MeH transformant for secreted expression of chimeric α-MeH protein with α-factor signal sequence (overexpressing both polypeptide precursor and glycosylated MeH forms), SG- another multicopy pPIC9K-MeH transformant for secreted expression of chimeric α-MeH, overexpressing glycosylated MeH form, Sc- pFGG3-MeH transformant of S. cerevisiae AH22 strain overexpressing full sequence MeH protein after induction with galactose. Solid arrows indicate unglycosylated MeH polypeptides, whereas dotted arrows - glycosylated MeH forms.

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