Figure 6From: Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiaeAnalysis of MeH in insoluble protein fractions from P. pastoris by Western blotting using anti-His antibody. The fractions insoluble in 1% TritonX-100 and 1M NaCl were obtained from P. pastoris transformants using the same method as described in Methods for S. cerevisiae samples (correspond to fraction 7 in Figure 3). VK indicates the sample obtained from P. pastoris transformant carrying one copy of pPIC3.5K-MeH expression cassete. Other markings are the same as in Figure 5 (dotted arrow indicates glycosylated MeH form, whereas solid arrow - unglycosylated MeH precursor).Back to article page