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Table 2 Validation of the predicted optimal culture conditions for optimal TS1-218 yield in one liter cultures.

From: Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Experiment

pH

Temp °C

MeOH %

Yield (mg)

Pre-Optimization

6

20

0.5

1.0 ± 0.14

Reference

7.5

15.5

1

15.3 ± 1.29

Optimized

7.1

11

1.2

21.4 ± 1.25

  1. Temp: temperature (°C); MeOH: methanol concentration (%, v/v). Results are derived from three different experimental runs. Statistical significant difference was observed between every experiment with regard to the final yields (p < 0.05).
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Microbial Cell Factories

ISSN: 1475-2859

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