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Figure 1 | Microbial Cell Factories

Figure 1

From: Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Figure 1

Coefficient plot of the ELISA ABS response. Coefficient plot with confidence intervals (error bars) showing the main (pH, Temp, MeOH concentration), squared (pH*pH, Temp*Temp, MeOH*MeOH) and interaction (pH*Temp, pH*MeOH) effects of the investigated factors on the ELISA absorbance response. The significance level was set at 95%. The significance of each term (factor) in the model is determined by the size of the coefficients (bars) and their error bars. Non-significant terms have an error bar that spans zero (p > 0.05). A negative coefficient for a main factor indicates that a decrease in that factor setting, relative to its center point, will have a positive effect on the yield of TS1-218 and consequently result in higher absorbance values. The interaction term temp*MeOH was not significant and was therefore removed from the model. The main term MeOH was also not significant, however, removing the term MeOH would also have removed the MeOH*MeOH and pH*MeOH terms which were significant.

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